Cldn1 Knockout cell line （CHO-K1）

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location： Home > Products > KO Cell Lines > Cldn1 Knockout cell line （CHO-K1）

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Cldn1 Knockout cell line （CHO-K1） Order

Cldn1 Knockout cell line （CHO-K1）
Catalog#
YKO-R075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cldn1 Knockout cell line （CHO-K1）
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Cldn1 Knockout cell line (CHO-K1)
Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:4
Species
chinese Hamster
Gene Name
Cldn1
Gene ID
100771737
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 1 (based on transcript Cldn1-201)
Validation: PCR+Sanger Sequencing
Cldn1 Knockout cell line （CHO-K1） Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cldn1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the CHO-K1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cldn1 gene knockout Cldn1 Knockout cell line (CHO-K1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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